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Diabetes mellitus and concurrent hypertension disorder are dreadful all over the world and are often managed by some drugs, such as metformin hydrochloride (MFH), enalapril maleate (ENM), and captopril (CAP). In this work, a reliable and fast quantitative analysis of these three components in tablets was carried out by Tchebichef image moment method and multivariate curve resolution with alternating least squares on three-dimensional (3D) spectra obtained by high-performance liquid chromatography coupled with photodiode array detection (HPLC-PAD). 3D spectra were obtained within only 2 min, and linear quantitative models were established by stepwise regression based on the calculated image moments. Among these two methods, Tchebichef image moment method showed outcome distinction. The correlation coefficients of cross-validation (RLoo-cv) are more than 0.988, while their recoveries are 100.1 ± 1.7% (MFH), 95.4 ± 5.4% (ENM), and 105.3 ± 5.7% (CAP), respectively. The intra- and inter-day precisions (RSD) are less than 5.42%. The proposed methods were also applied to the analysis of real tablets. This study reveals the effectiveness and convenience of the proposed image-moment method that may be a potential technology for the quality control and investigation of drugs in routine analysis.
A simple, rapid, and precise reversed-phase high-performance liquid chromatographic method for simultaneous analysis of metformin hydrochloride, pioglitazone hydrochloride, and glibenclamide in a tablet dosage form has been developed and validated. Chromatography was performed on a 25 cm × 4.6 mm i.d., 5-µm particle, C 18 column with 55:45 ( v/v ) acetonitrile - potassium dihydrogen phosphate buffer (pH adjusted to 3.0 š 0.1 with 5% orthophosphoric acid) as mobile phase at a flow rate of 1.5 mL min ?1 . UV detection was performed at 230 nm. Total run time was 10 min; metformin hydrochloride, pioglitazone hydrochloride, and glibenclamide were eluted with retention times of 1.362, 3.418, and 7.395 min, respectively. The method was validated for accuracy, precision, linearity, specificity, and sensitivity in accordance with ICH guidelines. Validation revealed the method is specific, rapid, accurate, precise, reliable, and reproducible. Calibration plots were linear over the concentration ranges 200-1000µg mL -1 for metformin hydrochloride, 200-1000 µg mL -1 for pioglitazone hydrochloride, and 50-300 µg mL -1 for glibenclamide. Limits of detection were 6.3, 15.4, and 8.2 ng mL -1 and limits of quantification were 19.09, 46.66, and 24.84 ng mL -1 for metformin hydrochloride, pioglitazone hydrochloride, and glibenclamide, respectively. The high recovery and low coefficients of variation confirm the suitability of the method for simultaneous analysis of the three drugs in tablets. The validated method was successfully used for quantitative analysis of triglycomate tablets.
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