In this work, the input-output method of dynamic parameters’ identification is experimentally tested. A method based on the transformation of a dynamic problem into a static problem by means of integration of the input and output signal was presented. The problem discussed in this article is the identification of the coefficients of stiffness matrices and eigenfrequencies of a discrete dynamic system subjected to kinematic input. The experimental analysis was carried out on a three-storey slab-and-column structure, which constitutes a physical model of a building. The vibrations of the model were excited kinematically by an earthquake simulator. The device has a computer-controlled, movable table top, which can move independently in three directions, that is, horizontally, vertically, and rotationally around the vertical axis. The aim of the experimental studies presented in this work was to determine the dynamic parameters of the model (stiffness, natural frequencies) using the input-output method in the time domain. Moreover, the results obtained with this method were compared with the results of experimental modal analysis (EMA) in order to verify their correctness. It was assumed that the movement of the base is horizontal and occurs in one direction. Two short-term, irregular kinematic excitations of the construction were considered, and the selected results and conclusions from experimental analyses were presented in this work.