A detailed revision of the brachiopods of the Lower-Middle Jurassic transition in the Lusitanian Basin (Andrade 2006) has enabled the establishment of the stratigraphical distribution of this fauna. More than 2,000 specimens were collected at 11 sections throughout the basin, including the Bajocian GSSP in Murtinheira (Cabo Mondego). In all, 24 species, belonging to 14 genera, have been recognized along a stratigraphical interval that includes the Upper Toarcian, the Aalenian, and the Lower Bajocian. The Toarcian associations are characterized by species also recorded in neighbouring basins, such as Stroudithyris stephanoides, Sphaeroidothyris vari, Pseudogibbirhynchia bothenhamptonensis and Soaresirhynchia renzi; as well as species endemic to the Lusitanian Basin, such as Choffatirhynchia alcariensis, Nannirhynchia delgadoi, N. cotteri, Praemonticlarella conimbriguensis, Neozeilleria duartei and Pamirorhynchia(?) jorali. This mixed palaeobiogeographical character persists in the Aalenian, in which the associations include, together with widely distributed species such as Neozeilleria anglica, Pseudogibbirhynchia mutans or Lophrothyris withingtonensis, other species known in neighbouring basins, such as Sphaeroidothyris uretae and Neozeilleria sharpei, and other species recorded only in the basin, such as Soaresirhynchia minor, S. murtinheirensis and Sphaeroidothyris henriquesae. In the Lower Bajocian, excluding Loboidothyris perovalis, only endemic species are present (belonging mainly to endemic genera), such as Lusitanina bituminis, Stroudithyris choffati, Lusothyris atlantica and Mondegia limica. The interpretation of these distributions also enables to propose a brachiopod based biozonation for the studied interval. Three zones have been erected: 1. the Renzi Zone, for the Upper Toarcian, with two subzones: Renzi and Duartei; 2. the Anglica Zone, that ranges from the Aalensis Biochronozone of the Toarcian to the base of the Bajocian. It has been subdivided in 3 subzones: Nuskae, Anglica and Uretae; 3. the Choffati Zone, which comprises the main part of the Discites, Laeviuscula and Sauzei biochronozones, with two subzones: Bituminis and Limica; 4. this proposal of biozonation can be correlated with other established in neighbouring basins, such as the Iberian Range in Spain or the French Basins.