Different stabilization devices have been used for treating lumbar spine disorders, including fusion, dynamic stabilization devices, flexible rods etc., which possess a different level of limitations. A simple experimental procedure is developed using a prototype lumbar spine specimen (L1-S), to evaluate the biomechanical performance of the lumbar spine. The range of motions (ROM) are tested for pedicle screw made of stainless steel (SS) fixation, using Teflon rod, ultra high molecular weight poly ethylene (UHMWPE) rod, poly ether ether ketone (PEEK) rod and SS flexible rod device (FRD). SS pedicle screw is used for fixation on the prototype lumbar spine. Experimental results are validated and compared with finite element (FE) results. It is observed that, in both flexion and extension, reduction in ROM is higher for Teflon and UHMWPE as compared to PEEK and FRD system. Differences between experimental and numerical results are found to be within an acceptable limit of 5–11%. For flexibility study, both numerical and experimental results support that PEEK rod plays an effective and important role among all the semi-rigid rods. The FRD devices are found to preserve the flexibility of the segment considerably better than PEEK rod.