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Acta Chromatographica

Tytuł artykułu

Assessing the efficacies of phenolic compounds in pomegranate juice using thin-layer chromatography

Autorzy Fakhri, E.  Petróczi, A.  Naughton, D. P. 
Treść / Zawartość
Warianty tytułu
Języki publikacji EN
EN Pomegranates (Punica granatum) have been known for centuries for their healing properties. The phenolic components of pomegranate are believed to be responsible for their antioxidant activities, hence playing a major role in reducing oxidative stress-related disease, such as cardiovascular diseases, cancer, and neurodegenerative diseases. In this study, thin-layer chromatography (TLC) was used to, first, separate and identify the phenolic constituents of pomegranate juice and, second, to assess the antioxidant efficacies of the identified compounds. Different oxidant and hydroxyl radical generating systems (Fe3+, Cu2+, H2O2, Fe2+-H2O2, and Cu2+-H2O2) were used in assessing the efficacies of phenolic compounds found in pomegranate juice. A 10 × 10 cm and 20 × 20 cm sized silica gel 60 F254 TLC plates with toluene-ethyl acetate-formic acid (60:40:10 v/v/v) as a mobile phase were used for the chromatographic separation. Two compounds, ellagic acid and gallic acid, were separated and identified. When pomegranate juice was challenged with the oxidant systems, it was observed that the phenolic compounds slowly disappeared in a concentration- and time-dependent manner. From the results, it was concluded that gallic acid had a higher antioxidant efficacy than ellagic acid. TLC has been applied for the first time to outline the antioxidant profile of pomegranate juice and assess the efficacies of phenolics using different oxidant systems, including redox-active metals and H2O2.
Słowa kluczowe
EN pomegranate juice   thin-layer chromatography   hydrogen peroxide   metal ions   antioxidants  
Wydawca University of Silesia in Katowice
Akademiai Kiado
Czasopismo Acta Chromatographica
Rocznik 2014
Tom Vol. 26, no. 3
Strony 563--573
Opis fizyczny Bibliogr. 19 poz., rys., tab.
autor Fakhri, E.
  • Kingston University School of Life Sciences Kingston upon Thames London KT1 2EE UK
autor Petróczi, A.
  • Kingston University School of Life Sciences Kingston upon Thames London KT1 2EE UK
autor Naughton, D. P.
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