The marine diatom cyclotella meneghiniana kűtzing as a producer of siderophore-like substances

• Autorzy: Cabaj A. , Kosakowska A.
• Języki publikacji: EN

Abstrakty

EN

Iron(III) plays an important role in biochemical processes occurring in plants and phytoplankton. Under iron deficiency (< 10-5 mol dm-3), most microorganisms activate a high-affinity iron acquisition system, producing and secreting iron(III)-specific chelators called siderophores. The aim of this study was to assess the influence of the ionic and complexed form of iron(III) on the production of siderophore-like substances by the marine diatom Cyclotella meneghiniana Kűtzing (SAG 1020-1a). The growth parameters measured were the number of cells and the content of chlorophyll a and protein. The production of siderophores was determined by non-specific (Cu-CAS) and specific (Csaky, Arnow) chemical tests and by biological tests with the bacteria mutants Morganella morganii SBK-3, Salmonella typhimurium enb-7 and Microbacterium flavescens JG-9. The results obtained show that both the concentration and physico-chemical form of iron(III) influence the number of cells and the content of chlorophyll a per cell, but not the content of protein per cell. The optimum concentration of Fe (10-5 mol dm-3) stimulated growth and chlorophyll a production, while iron stress in ionic form inhibited both of these. However, the enrichment of the medium with a complexing factor (EDTA) evidently promoted growth and chlorophyll a production. Siderophore-like substances were found to be active under both iron deficiency (in ionic and complexed forms) and sufficiency. It has been demonstrated that C. meneghiniana can produce hydroxamate-type siderophores and [alfa] -keto-[alfa]-hydroxyacids, but it probably does not produce catecholates.

Słowa kluczowe

EN

iron; siderophore-like substances; marine diatom

• Wydawca: Institute of Oceanography University of Gdańsk
• Czasopismo: Oceanological and Hydrobiological Studies
• Rocznik: 2005
• Tom: Vol. 34, No.1
• Strony: 57--72
• Opis fizyczny: Bibliogr. 72 poz., tab., wykr.

Twórcy

autor

Cabaj A.

• Institute of Oceanology, Polish Academy of Sciences, Powstańców Warszawy 55, 81-712 Sopot, Poland

autor

Kosakowska A.

• Institute of Oceanology, Polish Academy of Sciences, Powstańców Warszawy 55, 81-712 Sopot, Poland

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