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http://yadda.icm.edu.pl:80/baztech/element/bwmeta1.element.baztech-article-BPBB-0009-0001

Czasopismo

Acta of Bioengineering and Biomechanics

Tytuł artykułu

Opposite effects of electroporation of red blood cell membranes under the influence of zinc ions

Autorzy Kozlova. E.  Chernysh, A.  Moroz, V.  Sergunova, V.  Gudkova, O.  Fedorova, M.  Kuzovlev, A. 
Treść / Zawartość http://www.actabio.pwr.wroc.pl/acta.php
Warianty tytułu
Języki publikacji EN
Abstrakty
EN The goal of the study was to investigate the effects of zinc ions of various concentrations on the nanostructure of membrane of red blood cells in in vitro experiment. The suspension of red blood cells extracted from whole human blood was used. The calibrated electroporation and the atomic force microscopy (AFM) were used to analyse damage to membrane nanostructure. We studied the haemolysis after the electroporation at different zinc concentrations. A low concentration of zinc (0.15-0.5 mM) increased significantly the rate of haemolysis and reduced the residual level of non-haemolyzed cells. At high concentrations of zinc ions (0.5-10 mM), the rate constant was sharply reduced, at the same time the residual level increased. The relationship between haemoglobin coagulants and the zinc concentration was examined. High concentration of zinc caused haemoglobin aggregation. It was shown by AFM that the membrane nanostructure was essentially changed. It was experimentally established that there existed a special point of zinc concentration C = 0.5 +- 0.1 mM at which the course of the conjugate processes on the membranes of red blood cells was changed.
Słowa kluczowe
EN red blood cells   membrane   electroporation   zinc   AFM   competitive processes  
Wydawca Oficyna Wydawnicza Politechniki Wrocławskiej
Czasopismo Acta of Bioengineering and Biomechanics
Rocznik 2012
Tom Vol. 14, nr 1
Strony 3--13
Opis fizyczny Bibliogr. 17 poz., rys.
Twórcy
autor Kozlova. E.
autor Chernysh, A.
autor Moroz, V.
autor Sergunova, V.
autor Gudkova, O.
autor Fedorova, M.
autor Kuzovlev, A.
  • V. A. Negovsky Scientific Research Institute of General Reanimatology, Russian Academy of Medical Sciences, Moscow, Russia, waterlake@mail.ru
Bibliografia
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