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New application of mixed C18 -SCX SPE clean-up in gas chromatographic-mass spectrometric determination of six β 2 -agonist residues in pig's liver

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A new mixed C18-SCX SPE column and gas chromatography-mass spectrometry have been used for determination of six β 2-agonists residues in pig's liver. After hydrolysis, the six β 2-agonists were extracted with 6:4 (v/v) ethyl acetate-isopropanol and then cleaned on a commercial mixed-adsorbent solid-phase extraction column. The eluted solution was analyzed by gas chromatography-mass spectrometry (GC-MS) and quantified by the external standard method. Sample pre-treatment and GC-MS conditions were critically examined. The linear range was 0.005-2.00 mg L-1 and correlation coefficients were 0.9987-1.000. The LOD of mabuterol, clenbuteroland, and salbutamol was 0.2 µg kg -1. Average recoveries of these three compounds from spiked pig's liver ranged from 65.3 to 95.8% and relative standard deviations were between 5.4 and 9.9%. The LOD of cimaterol, bromobuterol, and ractopamine was 0.3 µg kg -1. Average recoveries of these three compounds from spiked pig's liver ranged from 63.8 to 88.1% and relative standard deviations were between 5.0 and 9.7%. These results indicate the method is highly sensitive, simple, inexpensive, and results in good purification. It could also meet requirements for analysis of pig's urine in domestic and import/ export inspection work.
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