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Determination of chloramphenicol residues in honey by monolithic column liquid chromatography-mass spectrometry after use of quechers clean-up

Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
An HPLC method with a monolithic column and detection by mass spectrometry has been established for determination of chloramphenicol in honey samples previously cleaned by use of a modification of the QuEChERS procedure. Honey samples were dissolved in water containing sodium chloride and extracted with acetonitrile. Further sample clean-up was performed by simple reversed-solid-phase dispersion with primary–secondary amine (PSA) adsorbent. Chloramphenicol (CAP) residues at ppb concentrations were detected by liquid chromatography–mass spectrometry (LC–MS), with electrospray ionization, in negative-ion mode. The mobile phase was methanol–0.2% aqueous ammonium acetate solution, 45:55 (v/v). Under these conditions CAP is eluted after ∼4 min and 8 min from a Merck RP-18e monolithic column and a conventional C18 column, respectively. Recovery at three fortification levels (0.2, 20, and 200 µg kg.1) was in the range 78–93% with RSD from 3.7 to 3.9%. The coefficient of determination, R2, was 0.9995 over a 0.1–100 µg L.1 linear range. Use of this method for determination of CAP in honey resulted in an LCL of 0.20 µg kg.1. The method was validated on the basis of EU decision 2002/657. CCá and CCâ for the honey matrix were 0.002 and 0.006 µg kg.1, so the method was fit for the purpose of monitoring commercial products or checking MRL compliance.
Słowa kluczowe
EN
Rocznik
Tom
Strony
320--327
Opis fizyczny
Bibliogr. 19 poz., rys., tab.
Twórcy
autor
autor
autor
autor
autor
  • College of Science, China Agricultural University, Beijing 100094, China
Bibliografia
  • [1] N.A. Botoglou and D.J. Fletouris, Anti-Bacterial Drugs: Drug Residues in Foods, Marcel Dekker, New York, 2001, pp. 85–87
  • [2] D.G. Jiiang and D.J. Yang, Chin. J. Food Hyg., 14, 44 (2002)
  • [3] J. Hua, T.Q. Yu, and Y.J. Sun, Anim. Sci. Vet. Med., 20, 36 (2003)
  • [4] Commission Regulation 1430/94 of 22 June 1994, Off. J. Eur. Commun., L156, 6 (1994)
  • [5] Council Directive 96/23/EC of 29 April 1996, Off. J. Eur. Commun., L125, 10 (1996)
  • [6] H.Y. Shen and H.L. Jiang, Anal. Chim. Acta, 535, 33 (2005)
  • [7] A.F. Forti, G. Campana, A. Simonella, et al., Anal. Chim. Acta, 529, 257 (2005)
  • [8] G. Scortichini, L. Annunziata, and M.N. Haouet, et al., Anal. Chim. Acta, 535, 43 (2005)
  • [9] M.J. Bogusz, H. Hassan, A.E. Eid, et al., J. Chromatogr. B, 807, 243 (2004)
  • [10] S. Impens, W. Reybroeck, J. Vercammen, et al., Anal. Chim. Acta, 483, 153 (2003)
  • [11] H.M. Ashwin, S.L. Stead, J.C. Taylor, et al., Anal. Chim. Acta, 529, 103 (2005)
  • [12] K. Vivekanandan, G.S. Madugundu, P. Sudhanand, et al., Rapid Commun. Mass Spectrom., 19, 3025 (2005)
  • [13] Commission Decision of 13 March 2003, Off. J. Eur. Commun., L71 (2003)
  • [14] K. Nakanishi, J. Porous Mater., 4, 67 (1997)
  • [15] M. Cledera-Castro, A. Santos-Montes, et al., J. Chromatogr. A, 1087, 57 (2005)
  • [16] A.H. Schmidt, J. Chromatogr. A, 1073, 377 (2005)
  • [17] K. Cabrera, D. Lubda, H.-M. Eggenweiler, et al., J. High Resolut. Chromatogr., 23, 93 (2000)
  • [18] M. Anastassiades, S.J. Lehotay, et al., J. AOAC Int., 86, 412 (2003)
  • [19] H.T. Ronning, K. Einarsen, et al., J. Chromatogr. A, 1118, 226 (2006)
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-article-BAT8-0006-0059
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