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Analysis of sugars by micellar liquid chromatography with UV detection

Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
A micellar liquid chromatographic procedure is described for si-multaneous determination of sugars previously derivatized with 4-amino-benzoic acid ethyl ester (4-ABEE). The optimized conditions, determined by use of the super-modified simplex method, were temperature 30°C, mo-bile phase containing 17.3 mM sodium dodecyl sulfate, 0.01 M phosphate buffer, pH 6.69, and 7.73% (v/v) ethanol, and UV detection at 307 nm. The 4-ABEE derivatives were separated in 10 min but a peak assigned to excess reagent appears at 13 min. Analytical data including linearity (r > 0.9987), limit of detection (35.47–81.74 µg mL-1), precision (RSD < 5.73%), and accuracy, measured by determination of recovery (>91%), support the use-fulness of the proposed method for measuring sugars. When the proposed method was applied to the analysis of sugars in an infant formula and a sample of sorghum syrup the results were in good agreement with the con-tent reported by manufacturer and with results obtained by refractive index detection.
Słowa kluczowe
EN
Rocznik
Tom
Strony
58--69
Opis fizyczny
Bibliogr. 25 poz., rys., tab.
Twórcy
autor
  • Department of Chemistry, University of Isfahan, Isfahan, 81746-73441, Iran
Bibliografia
  • [1] M. Peris-Tortajada in: L.M.L. Nollet (ed.) Handbook of Food Analysis, Marcel Dekker, New York, 1996; pp. 533–550
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  • [3] J.C.M. Waterval, H. Lingeman, A. Bult, and W.J.M. Underberg, Electrophoresis, 21, 4029 (2000)
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  • [6] S. Yasuno, T. Murata, K. Kukubo, T. Yamaguchi, and M. Kamei, Biosci. Biotechnol. Biochem., 61, 1944 (1997)
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  • [9] A. Berthod and M.C. Garcia Alvarez-Coque, Micellar Liquid Chromatography, Marcel Dekker, New York, 2000
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  • [11] J.R. Torres-Lapasio, R.M. Villanueva-Camanas, J.M. Sanchis-Mallols, M.J. Medina-Hernandez, and M.C. Garcia-Alvarez-Coque, J. Chromatogr. A, 677, 239 (1994)
  • [12] M.D. Rukhadze, G.S. Bezarashvili, M.V. Sebiskveradze, and V.R. Meyer, J. Chromatogr. A, 805, 45 (1998)
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  • [14] S.N. Deming and S.L. Morgan, Anal. Chem., 45, 278A (1973)
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  • [17] M.W. Routh, P.A. Swartz, and M.B. Denton, Anal. Chem., 49, 1422 (1977)
  • [18] W.T. Wang, N.C. Ledonne, B. Ackerman, and C.H.C. Sweeley, Anal. Biochem., 141, 366 (1984)
  • [19] V.R. Meyer, Practical High Performance Liquid Chromatography, Wiley, Chichester, 2000; pp. 45-47
  • [20] J.P. Foley and J.G. Dorsey, Anal. Chem., 55, 730 (1983)
  • [21] M.F. Borgerding, F.H. Quina, W.L. Hinze, J. Bowermaster, and H.M. McNair, Anal. Chem., 60, 2520 (1988)
  • [22] M.G. Khaledi, Anal. Chem., 60, 876 (1988)
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  • [24] J.C. Berridge and E.G. Morrissey, J. Chromatogr., 316, 69 (1984)
  • [25] G. Kuepper, Sweet Sorghum Production and Processing, The Kerr Center, Poteau, OK, 1992
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-article-BAT3-0037-0010
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