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HPLC with UV detection for measurement of vitamin E in human milk

Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Extraction of vitamin E from human milk, by use of hexane, with and without prior saponification, was compared. Analysis of milk extracts was performed by HPLC with a C18 column and UV detection. With both methods a significant relationship (P < 0.01) was obtained between levels of α-tocopherol and γ-tocopherol in human milk, although saponification resulted in higher and more consistent recovery of the internal standard, δ-tocopherol (99.6 ± 4.0%), and significantly improved the reliability of the data. The detection limit of the method (including saponification, extrac-tion, and HPLC analysis) was 0.65 µg mL-1 δ-tocopherol in milk. The pro-posed saponification step was a simple modification of the method, because it was performed in the same tube as the extraction, took 30 min only, and did not involve use of an inert gas. The improved method was used to measure vitamin E in several milk samples.
Słowa kluczowe
EN
Rocznik
Tom
Strony
48--57
Opis fizyczny
Bibliogr. 10 poz., rys., tab.
Twórcy
autor
autor
autor
  • Centre for Science and Technology in Medicine, School of Medicine, University of Keele, Thornburrow Drive, Hartshill, Stoke-on-Trent, ST4 7QB, UK
Bibliografia
  • [1] A.P. De Leenheer and A.E. Claeys, Clin. Chem., 25, 425 (1979)
  • [2] K.W. Miller, N.A. Lorr, and C.S. Yang, Anal. Biochem., 138, 340 (1984)
  • [3] G.L. Catignani and J.G. Bieri, Clin. Chem., 29, 708 (1983)
  • [4] J.E. Chappell, T. Francis, and M.T. Clandinin, Early Human Dev., 11, 157 (1985)
  • [5] J.E. Chappell, T. Francis, and M.T. Clandinin, Nutr. Res., 6, 849 (1986)
  • [6] A. Escriva, M.J. Esteve, R. Farre, and A. Frigola, J. Chromatogr. A, 947, 313 (2002)
  • [7] H.E. Indyk, Analyst, 113, 1217 (1988)
  • [8] E. Gimeno, E. Calero, A. Castellote, R.M. Lamuela-Raventos, and M.C. Lopez-Sabater, J. Chromatogr. A, 881, 255 (2000)
  • [9] B.R. Mendoza, S.M. Pons, A.I.C. Bargallo, and M.C. Lopez-Sabater, J. Chromatogr. A, 1018, 197 (2003)
  • [10] G.W. Chase, R.R. Eitenmiller, and A.R. Long, J. Liq. Chromatogr. Related Technol., 21, 2853 (1998)
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-article-BAT3-0037-0009
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