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Evaluating the toxicity of reactive dyes and dyed fabrics with the HaCaT cytotoxicity test

Wybrane pełne teksty z tego czasopisma
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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
We investigated the cytotoxicity of reactive dyes and dyed fabrics using human keratinocyte HaCaT cells in vitro. The HaCaT cells were exposed to three monochlortriazinyl dyes: yellow, red and blue with different concentrations. The HaCaT cells were also exposed to water extracts of dyed fabrics. After 72 hours exposure, the protein contents of the samples compared to the protein contents of non-exposed cells were measured. The level of protein content indicates the viability of the cells. The mean inhibitory concentration values (IC50) showed the dye concentration when the protein content of the sample was 50% of the protein content of the non-exposed cells. The mean inhibitory concentration values (IC20) when the protein content of the samples was 80% were also measured. The IC20 values show the limiting value of toxicity. The IC50 values show whether samples are clearly toxic. The IC50 value for the yellow dye was 237µg/ml and the IC20 value was 78µg/ml. The IC50 for the red dye was 155µg/ml: the red dye caused adverse effects under the lowest dye concentration (28µg/ml). The IC50 value for the blue dye was 278µg/ml and the IC20 value was 112µg/ml. Cotton fabrics dyed using these same three reactive dyes were extracted with water and the extracts were analysed using the HaCaT cell line. The viability of the cells was good, the protein content of the samples being over 80% compared to the non-exposed cells. The HaCaT cell test indicated the toxicity of pure dyes; the dyed fabrics had no adverse effect. The human keratinocyte HaCaT cells seem to be a useful tool for the study of the purity/toxicity of dyes and other substances applied to textiles.
Rocznik
Strony
217--223
Opis fizyczny
Bibliogr. 29 poz.
Twórcy
autor
autor
  • University of Huddersfield, Queensgate, Department of Design; tel: +44 1484472054 Huddersfield UK, j.s.pearson@hud.ac.uk
Bibliografia
  • 1. Assefa, Z., Garmyn, M., Bouillon, R., Merlevede, W., Vandenheede, J.R. & Agostinis, P. Differential stimulation of ERK and JNK activities by ultraviolet B irradiation and epidermal growth factor in human keratinocytes. Journal of investigative dermatology. 1997, 108 (6), 886-891.
  • 2. Birhanli, A. & Ozmen, M. Evaluation of toxicity and teratogenity of six commercial textile dyes using the frog embryo teratogenesis assay - Xenopus. Drug and Chemical Toxicologies. 2005, 28(1), 51-65.
  • 3. The Chemical Safety Data Sheets after 2001/58/EY; Drimarene yellow CL-2R, Drimarene blue CL- 2RL, Drimarene red CL-5B (Colour Index numbers CI: RR241, RY176, blue dye: number unknown )
  • 4. De Roos, A.J., Ray, R.M., Gao, D.L., Wernli, K.J., Fitzgibbons, E.D., Ziding, F., Astrakianakis, G., Thoma, D.B. & Checkoway, H. Colorectal cancer incidence among female textile workers in Shanghai, China: A Case -cohort Analysis of Occupational Exposures. Cancer Causes and Control, 2005, 16 (10), 1177-1188.
  • 5. Docker,A., Wattie, J.M., Topping, M.D., Luczynska, C.M., Newman Taylor, A.J., Pickering, C.A.C., Thomas, P.& Gompertz, D. Clinical and immunological investigations of respiratory disease in workers using reactive dyes. British Journal of Industrial medicine. 1987, 44 (8), 534-541.
  • 6. Dogan, E.E., Yesilada, E., Ozata, L. & Yologlu, S. Genotoxicity testing of four textile dyes in two crosses of Drosophila using wing somatic mutation and recombination test. Drug and Chemical Toxicologies. 2005, 28 (3), 289-301.
  • 7. Estlander, T. Allergic dermatoses and respiratory diseases from reactive dyes. Contact Dermatitis, 1988,18 (5), 290 - 297.
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  • 13. Kopponen P., Asikainen M., Törrönen R., Klemola K., Liesivuori J.& Kärenlampi S. In vitro cytotoxicity of textile dyes and extracts of dyed/ finished fabrics. Atla, 1997, 25, 539-546.
  • 14. Little, M.C., Gawkrodger, D.J. & MacNeil, S. Chromium and nickel- induced cytotoxicity in normal and transformed human keratinocytes. An investigation of pharmacological approaches to the prevention of Cr(VI)-induced cytotoxicity. British journal of dermatology, 1996, 134(2), 199-207.
  • 15. Manzini, B.M., Motolese, A., Conti, A., Ferdani, G. & Seidenari, S. Sensitization to reactive textile dyes in patients with contact dermatitis. Contact Dermatitis, 1996, 34 (3), 172-175.
  • 16. Mathur, N., Bathnagar, P., Nagar, P. & Bijarnia, M.K. Mutagenicity assessment of effluents from textile/dye industries of Sanganer, Jaipur (India): a case study. Ecotoxicology and Environmental safety. 2005, 61 (1), 105-113.
  • 17. Merryman, J.I. Effects of ultraviolet C radiation on cellular proliferation in p53-/-keratinocytes. Journal of environmental pathology toxicology and oncology, 1999, 18 (1), 1-9.
  • 18. Nilsson, R., Nordlinder, R., Wass, U., Meding, B. & Belin, L. Asthma, rhinitis, and dermatitis in workers exposed to reactive dyes. British Journal of Industrial Medicine. 1993, 50 (1),65-70.
  • 19. O`Reilly, J.P. & Mothersill, C. Comparative effects of UV A and UV B on clonogenic survival and delayed cell death in skin cell lines from humans and fish. International journal of radiation biology, 1997, 72 (1), 111-119.
  • 20. Park, H.S., Lee, M.K., Kim, B.O., Lee, K.J., Roh J.H., Moon, Y.H. & Hong C-S. Clinical and immunologic evaluations of reactive dye-exposed workers. Journal of Allergy and Clinical Immunology. 1991, 87 (3), 639-649.
  • 21. Przybojewska B., Baranski B., Spiechowicz E. & Szymczak W. Mutagenic and genotoxic activity of chosen dyes and surface active compounds used in the textile industry. Polish Journal of Occupational medicine, 1989, 2 (2), 171-185.
  • 22. Shimizu, H., Banno, Y., Sumi, N., Naganawa, T., Kitajima, Y. & Nozawa, Y. Activation of p38 mitogen-activated protein kinase and caspases in UVB-induced apoptosis of human keratinocyte HaCaT cells. Journal of investigative dermatology, 1999, 112 (5), 769-774.
  • 23. Thoren, K., Meding, B., Nordlinder, R. & Belin, L. Contact dermatitis and asthma from reactive dyes. Contact Dermatitis, 1980, 15 (3), 186.
  • 24. Trotman, E.R.. Dyeing and chemical technology of textile fibres. Charles Griffin & Co Ltd, Worcester 1984.
  • 25. Wang,C., Yediler, A., Lienert, D., Wang, Z. & Kettrup, A. Toxicity evaluation of reactive dyestuffs, auxiliaries and selected effluents in textile finishing industry to luminescent bacteria Vibrio fischeri. Chemosphere, 2002, 46 (2), 339-344.
  • 26. Wilhelm, K-P., Samblebe, M., Siegers, C-P.Quantitative in vitro assessment of N-alkyl sulphate induced cytotoxicity in human keratinocytes (HaCaT). Comparison with in vivo human irritation tests. British journal of dermatology, 1994, 130 ( 1), 18-23.
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  • 29. Öko-Tex Standard 100, Textilveredlung 32 ( 1997), Nr 7/8.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-4384ebbf-44c3-4d47-8d33-222893a8b296
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