Narzędzia help

Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
first last
cannonical link button

http://yadda.icm.edu.pl:80/baztech/element/bwmeta1.element.baztech-3f66d7d4-df99-4e87-a076-564d73751c6e

Czasopismo

Acta Chromatographica

Tytuł artykułu

Target-guided isolation and purification of antioxidants from Elsholtzia densa benth. var. densa by DPPH antioxidant assay and dual-mode HSCCC

Autorzy Zhou, W. N.  Ouyang, J.  Wang, Z. H.  Wang, X. Y.  Suo, Y. R.  Zhang, Z.  Wang, H. L. 
Treść / Zawartość http://akjournals.com/view/journals/1326/1326-overview.xml http://acta-chromatographica.us.edu.pl/
Warianty tytułu
Języki publikacji EN
Abstrakty
EN Elsholtzia densa Benth. var. densa (Lamiaceae) is a famous medicinal herb which has been widely used for treatment of colds, headaches, pharyngitis, fever, diarrhea, digestion disorder, rheumatic arthritis, nephritises, and nyctalopia in China. In this study, fraction of the ethyl alcohol extract of E. densa (aerial part) by different polarity solvents indicated that the ethyl acetate soluble fraction exhibited a potent 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity with the IC50 value of 148.2 μg/mL. Under the target guidance of DPPH experiment, isoquercitrin, trachelogenin, ethyl caffeate, and arctigenin were separated with purities 95.98%, 92.98%, 96.07%, and 88.83%, respectively, by a dual-mode high-speed counter-current chromatography (HSCCC) method using n-hexane–ethyl acetate–methanol–water (4.5:5:3:4, v/v/v/v) as the solvent system. In order to evaluate the scientific basis, antioxidant activity of four isolated compounds was assessed by the radical scavenging effect on DPPH radical; isoquercitrin and ethyl caffeate showed stronger antioxidant activities with IC50 values of 9.4 μg/mL and 9.2 μg/mL, respectively, while trachelogenin and arctigenin showed weak antioxidant activities with IC50 values of >500 μg/mL and 72.8 μg/mL, respectively. Results of the present study indicated that the combinative method using DPPH antioxidant assay and dual-mode HSCCC could be widely applied for rapid screening and isolating of antioxidants from complex traditional Chinese medicine extract.
Słowa kluczowe
EN Elsholtzia densa Benth   var   densa   antioxidant   target-guided   dual-mode HSCCC  
Wydawca University of Silesia in Katowice
Akademiai Kiado
Czasopismo Acta Chromatographica
Rocznik 2018
Tom Vol. 30, no. 3
Strony 180--185
Opis fizyczny Bibliogr. 33 poz.
Twórcy
autor Zhou, W. N.
  • Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China
  • University of the Chinese Academy of Sciences, Beijing 100049, China
autor Ouyang, J.
  • Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China
  • University of the Chinese Academy of Sciences, Beijing 100049, China
autor Wang, Z. H.
  • College of Life Sciences, Yantai University, Yantai 264025, China
autor Wang, X. Y.
  • Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China
autor Suo, Y. R.
  • Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China
autor Zhang, Z.
  • JALA Co. Ltd., Shanghai 200233, China
autor Wang, H. L.
  • Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China, hlwang@nwipb.cas.cn
Bibliografia
[1] Wu, Z. Y.; Li, X. W. Flora of China, Science Press: Beijing, 1977, volume 66.
[2] Harley, R. M.; Atkins, S.; Budantsev, A. L.; Cantino, P. D.; Conn, B. J.; Grayer, R.; Harley, M. M.; De Kok, R.; Krestovskaja, T.; Morales, R. Labiatae, The Families and Genera of Vascular Plants, Flowering Plants. Dicotyledons, Springer, 2004, 167–275. https://www.infona.pl/resource/bwmeta1.element.springer-327c6c7e-7a02-300c-9f1a-89e35c139927.
[3] Sun, L.; Yin, Z.; Fu, Z.; Zheng, S.; Shen, X. Acta Bot. Sin. 1995, 38, 672–676.
[4] Thappa, R.; Agarwal, S.; Kapahl, B.; Srivastava, T. J. Essent. Oil Res. 1999, 11, 97–103.
[5] Zhao, Y.; Zhao, Y.; Cheng, Y.-G. Journal of Yunnan Normal University (Natural Sciences Edition) 2005, 1: 009.
[6] Wu, B.; Zoriy, M.; Chen, Y.; Becker, J. S. Talanta 2009, 78, 132–137.
[7] Vashist, V.; Atal, C. Cell. Mol. Life Sci. 1970, 26, 817–818.
[8] Melkani, A. B.; Beauchamp, P. S.; Dev, V.; Whalen, C.; Mathela, C. S. J. Essent. Oil Res. 1994, 6, 475–479.
[9] Liu, Y.; Si, J.-Y.; Cao, L.; Jia, X.-G.; Li, X.-J. Natural Product Research and Development 2012, 24, 1070–1074.
[10] Wang, Y.; Lee, S. M. Y.; Liu, A. , Elsholtzia: Review of Traditional Uses, Chemistry and Pharmacology, 2007, 16, 73.
[11] Uwai, K.; Osanai, Y.; Imaizumi, T.; Kanno, S.-i.; Takeshita, M.; Ishikawa, M. Bioorg. Med. Chem. 2008, 16, 7795–7803.
[12] Guo, Z.; Liu, Z.; Wang, X.; Liu, W.; Jiang, R.; Cheng, R.; She, G. Chem. Cent. J. 2012, 6, 147.
[13] Xue, X.-J.; Guo, Z.-J.; Zhang, H.; Liu, X.; Luo, J.; Li, D.-D.; Li, J. Nat. Prod. Res. 2016, 1–5.
[14] Borek, C. J. Nutr. 2001, 131, 1010S-1015S.
[15] Zhang, Y.; Shi, S.; Wang, Y.; Huang, K. J. Chromatogr. B 2011, 879, 191–196.
[16] Still, W. C.; Kahn, M.; Mitra, A. J. Org. Chem. 1978, 43, 2923–2925.
[17] Tang, D.-S.; Zhang, L.; Chen, H.-L.; Liang, Y.-R.; Lu, J.-L.; Liang, H.-L.; Zheng Sep, X.-Q . Purif. Technol. 2007, 56, 291–295.
[18] Harada, K.-I.; Suzuki, M.; Dahlem, A. M.; Beasley, V. R.; Carmichael, W. W.; Rinehart, K. L. Toxicon 1988, 26, 433–439.
[19] Béress, A.; Wassermann, O.; Bruhn, T.; Béress, L.; Kraiselburd, E. N.; Gonzalez, L.V.; de Motta, G. E.; Chavez, P. I. J. Nat. Prod. 1993, 56, 478–488.
[20] Hostettmann, K.; Wolfender, J.-L.; Terreaux, C. Pharm. Biol. 2001, 39, 18–32.
[21] Zhao, C.; He, C. J. Sep. Sci. 2006, 29, 1630–1636.
[22] Ito, Y. J. Chromatogr. A 2005, 1065, 145–168.
[23] Ito, Y. J. Chromatogr. A 1991, 538, 3–25.
[24] Agnely, M.; Thiebaut, D. J. Chromatogr. A 1997, 790, 17–30.
[25] Li, H.-B.; Chen, F. J. Chromatogr. Sci. 2009, 47, 337–340.
[26] Tapia, A.; Rodriguez, J.; Theoduloz, C.; Lopez, S.; Feresin, G. E.; Schmeda-Hirschmann, G. J. Ethnopharmacol. 2004, 95, 155–161.
[27] Pérez-Bonilla, M.; Salido, S.; van Beek, T. A.; Linares-Palomino, P. J.; Altarejos, J.; Nogueras, M.; Sánchez, A. J. Chromatogr. A 2006, 1112, 311–318.
[28] Sun, L.-N.; Zhang, Y.-F.; He, L.-Y.; Chen, Z.-J.; Wang, Q.-Y.; Qian, M.; Sheng, X.-F. Bioresour. Technol. 2010, 101, 501–509.
[29] Alvi, K. A. J. Liq. Chromatogr. Relat. Technol. 2001, 24, 1765–1773.
[30] Brand-Williams, W.; Cuvelier, M.-E.; Berset, C. LWT--Food Sci. Technol. 1995, 28, 25–30.
[31] Harborne, J. B. Prog. Clin. Biol. Res. 1985, 213, 15–24.
[32] Halliwell, B. Free Radical Res. Commun. 1990, 9, 1–32.
[33] Valentová, K.; Vrba, J.; Bancířová, M.; Ulrichová, J.; Křen, V. Food Chem. Toxicol. 2014, 68, 267–282.
Uwagi
PL Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2018).
Kolekcja BazTech
Identyfikator YADDA bwmeta1.element.baztech-3f66d7d4-df99-4e87-a076-564d73751c6e
Identyfikatory
DOI 10.1556/1326.2017.00192